Figure 4

Heatmap of genes in putative drug-resistant cross-talks in breast cancer cell-line: BT474 (GSE16179). Heatmap image of comparative gene expression changes of parental and resistant conditions in (A) all 78 genes in all 83, 133 and 277 putative drug-resistant cross-talks using signaling pathways from Reactome, KEGG and WikiPathway database, respectively, and (B) 37 selected genes based on their differential regulation. Here, for each gene, the expression value at each of the 4 conditions (2 parental conditions, and 2 resistant conditions) is the average value of 3 sample patients [16]. For each gene, these 4 expression values (each of them is the average of 3 samples) were transformed into z-scores (zero mean, unit standard deviation) and each z-score was normalized with the maximum absolute value of the z-scores across that particular gene. For both (A) and (B), red and green bars indicate up-regulation and down-regulation, respectively.