Figure 5

Prediction of the endogenous TGN. Prediction of protein amounts, fluxes and regulatory influences of the endogenous TGN. Model A is simulated multiple times using a representative parameter sample from the posterior distribution with all experimental inputs inactive. The simulation represents the endogenous Golgi of an average cell. Units for nodes are molecules per cell and flow rates are given in molecules per hour. Mini diagrams next to the nodes depict the steady state distributions of the endogenous abundances with expectation values and 0.5% and 99.5% confidence intervals on a logarithmic scale. Color encoding of nodes and edges represent expectation values of abundances and flow rates. In the case of PKD there are two activation reactions (basal and CERT dependent). Catalytic influences are represented with dashed arrows and are modeled with Michaelis Menten similar kinetics. Expectation values of the operating points for these regulations are given in units of the respective maximum reaction rate. Values far below v _max depict non-saturated coupling.