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Table 1 Measured and calculated cultivation data for P. fluorescens CLEs

From: Fluxome study of Pseudomonas fluorescens reveals major reorganisation of carbon flux through central metabolic pathways in response to inactivation of the anti-sigma factor MucA

Strain

OD 660

Specific fructose uptake [mmolC/gDW h]

Specific alginate production [mmolC/ gDW h]

Specific biomass production [mmolC/gDW h] 3)

Specific CO 2 excretion [mmol/gDW h] 4)

Y xs [%]

wild-type1)

8.0±0.1

11.0±0.6

0.0

1.4±0.13

9.6±0.61

12

wild-type2)

7.0±0.1

11.8±0.6

0.0

1.4±0.13

10.4±0.61

12

Estimated5)

 

11.76±0.54

 

1.34±0.13

10.41±0.55

 

mucA-ΔalgC 2)

8.9±0.1

7.34±0.4

0.0

1.4±0.13

6.0±0.42

19

Estimated5)

 

7.39±0.32

 

1.30±0.13

6.08±0.34

 
  1. 1)Experimental design cultivation on wild type. 2)Main experiment on both strains. 3)Calculated from culture growth rate and biomass composition based on biomass equation. 4)Difference between fructose uptake and biomass production. No additional carbon-containing products (i.e., organic acids) were detected in the culture supernatant. 5) Estimated rates from simulation. Standard deviations are estimated from the process data and used for the 13C modelling.
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BMC Systems Biology

ISSN: 1752-0509

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