AMPK phosphorylation of AKT2 at Serine 268. (A) Schematic of AMPK regulation of EC migration through AKT2 phosphorylation. (B) Bottom panel represents in vitro kinase assay using (γ-32P) ATP and full-length recombinant AKT2 in the presence or absence of AMPK. Top panel represents CB staining for equal loading of recombinant AKT2. (C,D) CPM quantification of kinase assay using AKT2 Ser268 and Ser268A peptides (C) or full-length AKT2 (D). The experimental conditions were the same as those in Figure. 2. Student t-test used to determine *p < 0.5, all experiments were repeated at least 3 times.