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Figure 6 | BMC Systems Biology

Figure 6

From: Robust network structure of the Sln1-Ypd1-Ssk1 three-component phospho-relay prevents unintended activation of the HOG MAPK pathway in Saccharomyces cerevisiae

Figure 6

Growth defects following massive overexpression of phospho-relay components are due to activation of the HOG pathway. A We assayed for Hog1 phosphorylation after overexpression of relay components (Sln1, Ypd1, Ssk1) and positive controls (Pbs2, Ssk22). The parental strain carrying the empty plasmid vector was used as a negative control. B We quantified the amount of phosphorylated Hog1 (relative to Hog1) in five biological replicates of this experiment. Error bars represent the standard error. Pbs2, Ssk22, and Ssk1 caused a significant (∗) change in Hog1 phosphorylation levels after overexpression for 30 minutes (p=0.0395, 0.0096, and 0.0224, respectively; paired t-test). Hog1 phosphorylation levels were also significantly lower in the Ypd1 overexpression strain (p=0.0246, two-way ANOVA).

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BMC Systems Biology

ISSN: 1752-0509

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