Figure 7

Underexpression of Ypd1 causes a severe growth defect. A diploid homozygous GEV strain carrying one inducible allele of Ypd1 (YPD1/P _{G A L1}-YPD1) was sporulated onto 10 nM β-estradiol, and individual spores were frogged onto plates containing different concentrations of β-estradiol. At low concentrations of β-estradiol, spores carrying the inducible allele (P _{G A L1}-YPD1, row 2 and row 4) exhibited a growth defect due lower levels of Ypd1 and hyperactivation of the HOG pathway. Spores carrying the wild-type allele (row 1 and row 3) showed no growth defect.