Fig. 8

Fit and predictions obtained on a single realization of data in the Sln1 scheme. Black and red points (connected by lines of the same color) refer to data points which were used for parameter estimation and validation, respectively. In this example, all doses of Sln1 data and the data on the downstream species (protein, mRNA and internal glycerol) were used for parameter estimation. The magenta lines show the profiles obtained (both fit and prediction) by using the true model for the parameter estimation. The orange lines belong to the profiles obtained by the simplified model structure. All concentrations are given in percentages. The top three rows are for the Hog1PP data. The titles for each graph show the dose and the cell type related to the experiment in which the Hog1PP data was collected. The last row of graphs give the concentration ratios for the downstream species. The associated data was collected in a single experiment with WT cells following a 0.5 M. NaCl shock