Table 2 Comparison of metabolic engineering targets identified by various reaction-centric approaches and its equivalents in FSA for succinate overproduction in E. coli
From: Flux-sum analysis identifies metabolite targets for strain improvement
Reaction-centric algorithm | FSA | ||
|---|---|---|---|
Algorithm | # interventions | Targets | Equivalent target |
OptKnock | Two | PFL (X), LDH (X) | PFL (X) corresponds to formate (↓) |
|  | Three | ALCD (X), PFL (X), LDH (X) | PFL (X) corresponds to formate (↓) |
|  |  | ALCD (X), PTA (X), ACK (X) | PTA (X) and ACK (X) corresponds to acetate (↓) |
|  | Four | ALCD (X), PTA (X), ACK (X), PYK (X) | PTA (X) and ACK (X) correspond to acetate (↓). PYK (X) corresponds to pyruvate (↓) |
|  |  | ALCD (X), PTA (X), ACK (X), TKT (X) | PTA (X) and ACK (X) correspond to acetate (↓) |
OptReg | Two | PFL (X), PPC (↑) | PFL (X) corresponds to formate (↓) |
|  | Three | PFL (X), PPC (↑), ALCD (↓) | PFL (X) corresponds to formate (↓) |
|  | Four | PPC (↑), CS (↑), PDH (↓), ALCD (↓) | CS (↑) possibly correspond to isocitrate (↑) |
OptForce | Two | PPC (↑), CS (↑) | CS (↑) possibly correspond to isocitrate (↑) |
OptForce | Three | PPC (↑), CS (↑), MDH (↓) | CS (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑) |
|  |  | PPC (↑), ACONT (↑), MDH (↓) | ACONT (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑) |
OptForce | Four | PPC (↑), CS (↑), MDH (↓), PFL (↓) | CS (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑). PFL (↓) corresponds to formate (↓) |
|  |  | PPC (↑), ACONT (↑), MDH (↓), PFL (↓) | ACONT (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑). PFL (↓) corresponds to formate (↓) |