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Table 2 Comparison of metabolic engineering targets identified by various reaction-centric approaches and its equivalents in FSA for succinate overproduction in E. coli

From: Flux-sum analysis identifies metabolite targets for strain improvement

Reaction-centric algorithm FSA
Algorithm # interventions Targets Equivalent target
OptKnock Two PFL (X), LDH (X) PFL (X) corresponds to formate (↓)
  Three ALCD (X), PFL (X), LDH (X) PFL (X) corresponds to formate (↓)
   ALCD (X), PTA (X), ACK (X) PTA (X) and ACK (X) corresponds to acetate (↓)
  Four ALCD (X), PTA (X), ACK (X), PYK (X) PTA (X) and ACK (X) correspond to acetate (↓). PYK (X) corresponds to pyruvate (↓)
   ALCD (X), PTA (X), ACK (X), TKT (X) PTA (X) and ACK (X) correspond to acetate (↓)
OptReg Two PFL (X), PPC (↑) PFL (X) corresponds to formate (↓)
  Three PFL (X), PPC (↑), ALCD (↓) PFL (X) corresponds to formate (↓)
  Four PPC (↑), CS (↑), PDH (↓), ALCD (↓) CS (↑) possibly correspond to isocitrate (↑)
OptForce Two PPC (↑), CS (↑) CS (↑) possibly correspond to isocitrate (↑)
OptForce Three PPC (↑), CS (↑), MDH (↓) CS (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑)
   PPC (↑), ACONT (↑), MDH (↓) ACONT (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑)
OptForce Four PPC (↑), CS (↑), MDH (↓), PFL (↓) CS (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑). PFL (↓) corresponds to formate (↓)
   PPC (↑), ACONT (↑), MDH (↓), PFL (↓) ACONT (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑). PFL (↓) corresponds to formate (↓)
  1. Results of OptKnock, OptReg and OptForce are reproduced from Ranganathan et al. [19]. X - Deletion, ↑ - Upregulation or Intensification and ↓ - Downregulation or Attenuation