From: Flux-sum analysis identifies metabolite targets for strain improvement

Reaction-centric algorithm | FSA | ||
---|---|---|---|

Algorithm | # interventions | Targets | Equivalent target |

OptKnock | Two | PFL (X), LDH (X) | PFL (X) corresponds to formate (↓) |

Three | ALCD (X), PFL (X), LDH (X) | PFL (X) corresponds to formate (↓) | |

ALCD (X), PTA (X), ACK (X) | PTA (X) and ACK (X) corresponds to acetate (↓) | ||

Four | ALCD (X), PTA (X), ACK (X), PYK (X) | PTA (X) and ACK (X) correspond to acetate (↓). PYK (X) corresponds to pyruvate (↓) | |

ALCD (X), PTA (X), ACK (X), TKT (X) | PTA (X) and ACK (X) correspond to acetate (↓) | ||

OptReg | Two | PFL (X), PPC (↑) | PFL (X) corresponds to formate (↓) |

Three | PFL (X), PPC (↑), ALCD (↓) | PFL (X) corresponds to formate (↓) | |

Four | PPC (↑), CS (↑), PDH (↓), ALCD (↓) | CS (↑) possibly correspond to isocitrate (↑) | |

OptForce | Two | PPC (↑), CS (↑) | CS (↑) possibly correspond to isocitrate (↑) |

OptForce | Three | PPC (↑), CS (↑), MDH (↓) | CS (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑) |

PPC (↑), ACONT (↑), MDH (↓) | ACONT (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑) | ||

OptForce | Four | PPC (↑), CS (↑), MDH (↓), PFL (↓) | CS (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑). PFL (↓) corresponds to formate (↓) |

PPC (↑), ACONT (↑), MDH (↓), PFL (↓) | ACONT (↑) and MDH (↓) possibly correspond to glyoxylate (↑) and isocitrate (↑). PFL (↓) corresponds to formate (↓) |