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Fig. 2 | BMC Systems Biology

Fig. 2

From: Mathematical analysis of the regulation of competing methyltransferases

Fig. 2

Binding of 5mTHF to GNMT. Panel a shows the concentrations of the three substrates, free GNMT(blue curve), G N M T-5m T H F(red), and 5m T H F-G N M T-5m T H F(green) as a function of the concentration of 5m T H F. Total GNMT concentration is 1 μM. Panel b shows the concentration of bound 5m T H F as a function of total 5m T H F. The open circles are data replotted from [17], Fig. 2, and the blue curve is the model calculation. The concentration of GNMT is 1 μM. Since the concentration of bound 5m T H F goes well above 1 μM, this proves conclusively that two 5mTHF can bind to each G N M T. The data (open circles) in Panel c, taken from [17], Fig. 3, shows how the activity of GNMT decreases from 1 (normal) as the concentration of 5mTHF increases. The red curve shows the activity of GNMT, as computed by the model, if one assumes that once and twice bound GNMT lose all activity. The green curve shows the activity of GNMT as computed by the model if one assumes that once and GNMT has 50 % activity and twice bound GNMT has no activity. The green curve gives an excellent fit to the data

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