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Table 2 Comparison of methods to regulate FBA

From: FlexFlux: combining metabolic flux and regulatory network analyses

Method Associated software Requires Dynamic Steady-state Multistate Regulatory Network
   external data analysis analysis support loops support inference
RSA + FBA Yes (FlexFlux) No No Yes Yes Yes No
RFBA Yes (COBRA and FlexFlux) No Yes No COBRA: No FlexFlux: Yes Yes No
SRFBA No No No Yes No No No
iFBA No No Yes No No Yes No
PROM No Yes Yes No No Yes Yes