Fig. 3

Expression of cytokines in GI tract tissue. Mice were orally inoculated with WT C. albicans and sacrificed 20 days later. Additional mice did not receive C. albicans inoculation and were sacrificed either on the day of inoculation (day 0) or on day 20. Expression of cytokines IL-17A (panel a) and IL-22 (panel b) in stomach tissue was measured by qRT-PCR and normalized using expression of GAPDH. Geometric mean for colonized mice was set to 1. Panels a and b: Circles, uninoculated, day 0; squares, uninoculated, day 20; filled diamonds, C. albicans colonized, day 20. Grey circles, samples with undetectable signal. Bar shows the geometric mean. **, p < 0.0001 (t test with log-transformed data)