Fig. 2

Model predictions for the half-maximal effective ligand concentrations as a function of the number of binding sites and ligand concentration. a. The dependence of the half-maximal effective ligand concentration, U ^0.5₀ /K and U ^0.5 _n /K, for the apo- and saturated multisite protein conformations respectively, on the number of binding sites. The effect of the increasing of the amount of binding sites is negligible for the fully bound conformation. b. Calculations for the half-width between the half-maximal effective ligand concentrations as a function of the ligand concentration for proteins with two, three, four and five binding sites. c. The difference between ligand concentrations for the saturated multisite protein conformations when the protein species equal to 90 % and 10 % of the total concentration as a function of the ligand concentration for the proteins with one to six binding sites. d. The difference between ligand concentrations for the saturated multisite protein conformations when the protein species equal to 90 % and 10 % of the total concentration as a function of the number of binding sites up to six