Fig. 3
Temporal characteristics of the apo- and fully bound species in response to a ligand jump. The dynamics of the concentration of proteins was investigated for apo- (a) and fully bound (b) forms in response to the non-dimensional ligand concentration change from U 0/K = 0.1 to U 1/K = 10 as a function of non-dimensional time η = t ⋅ k −. The dotted lines indicate the time, τ 0.50 , required for the non-dimensional concentration of apo- conformation, N 0/L T , to reach half of the fall in concentration and the period of time, τ 0.54 , that takes for the fully saturated protein species, N 4/L T , to gain half of the growth in concentration. These kinetic parameters then were subject to the investigation as a function of the initial (c) and final (d) ligand concentrations. The presented analysis clearly demonstrates the bell shaped dependence of τ 0.54 on the final ligand concentration