Fig. 4

Characteristic time required for intermediate conformations to reach their maximum levels as a function of the step change magnitude. The analysis shows that the non-dimensional time (η ^max _m  = τ ^max _m k ⁻, where m = 1, 2 and 3) required for reaching the maximum level of the intermediate species, is inversely proportional to the concentration of the applied ligand U ₁/K. This effect is due to the growing abundance of the free ligand concentration available for faster interaction with the multisite protein