Fig. 2

Comparison between simulated and experimental data. Comparison between experimental concentration (points) and normalized model predictions (lines) for pAkt-S473, ppERK1/2, pmTOR-S2448 and pP70S6K-T389. The profile of ppERK1/2-Y202,Y204 obtained by increasing the strength of the feedback between ERK and GRB2/SOS is shown in dotted lines. Values are reported for experimental data of human skeletal muscle cells (SkMCs) exposed to EBSS + 100 nM insulin at time 0′, 2′, 5′, 10′, 30′, and 60′. All measurements were taken in three biological replicated, and for each biological replicates, three technical replicated measurements were taken. All data are expressed in arbitrary units (AU) and rescaled between 0 and 1 for sake of comparison