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Table 1 Parametric sensitivity analysis of the complete model for GLUT4 membrane translocation

From: A rule-based model of insulin signalling pathway

Parameter Complete model wo_feedback p70S6K wo_feedback
ERK1/2
Process
k_13 −84.89 % −83.59 % −84.89 % GLUT4 translocation
k13p 66.02 % 66.76 % 66.02 % GLUT4 translocation
k13 19.03 % 17.01 % 19.03 % GLUT4 translocation
n_p70 −15.21 % - −15.21 % p70 mediated IRS1 phosphorylation at Ser
k9a 8.25 % 13.87 % 8.25 % lipids PI(3,4,5)P3 formation
k9s −8.25 % −13.86 % −8.25 % lipids PI(3,4,5)P3 formation
k8 7.36 % 10.88 % 7.36 % IRS1_PI3K complex formation
k_8 −7.36 % −10.87 % −7.35 % IRS1_PI3K complex formation
k_14 −5.25 % −5.19 % −5.25 % GLUT4 degradation
k_7 −5.05 % −2.68 % −5.05 % IRS1 dephosphorylation at Tyr
k7 5.05 % 2.68 % 5.05 % IRS1 phosphorylation at Tyr
k_7p 4.45 % 0.53 % 4.45 % IRS1 dephosphorylation at Ser
k15 −4.30 % - −4.30 % IRS1 phosphorylation by P70S6K
Kd_p70 0.72 % - 0.72 % p70 mediated IRS1 phosphorylation at Ser
Vmax 0.65 % 2.18 % 0.65 % PKC and p70 mediated IRS1 phosphorylation at Ser
k4p −0.51 % −0.27 % −0.52 % Phosphorylated receptor internalization
k_21 −0.18 % - −0.18 % TSC1-TSC2 T1462_phosphorylation_by_Akt_pT309
k7p −0.15 % −0.54 % −0.15 % IRS1 phosphorylation at Ser
k41 0.14 % 0.46 % 0.14 % IRS1-GS and IRS1-SHP2 complex disruption
  1. Sensitivity coefficients are ranked accordingly to their absolute values and their corresponding values upon P70S6K-IRS1 and ERK1/2-GRB2/SOS negative feedback loop removal are also shown. Only coefficient greater than 0.1 % (absolute value) either in the original or modified model are reported. The column “Process” describes the biological process to which the parameter takes place