Fig. 2

Gal80 is able to create a shuttling system. a Average curves of lacZ mRNA expression at the mid-saggittal plane in embryos with no gal80 (n = 27), two copies of UASx5:gal80 (n = 36), two copies of UASx3:gal80 (n = 13), or one copy of UASx5:gal80 (n = 22), from mothers with two copies of Gal4GCN4. Individual curves for each embryo are shown with the average in Additional file 1: Figure S2. b Box plots of AP coordinate where normalized intensity is 0.31 (see dashed lines in [a], maximum difference between no gal80 control and two copies of UASx5:gal80 [c]). Asterisk denotes statistical significance (p < 0.005). c Difference between normalized intensity of lacZ without gal80 versus with varying amounts of gal80, dashed line denotes p = 0.05