Fig. 3

Susceptibility and CD163 dynamics. Batch specific least square mean (LSM) percentages and standard errors obtained from the experimental data (a–c) and from the predictions of model B (d–f) obtained by linear mixed model analysis. Batches 1 and 3 consisted of three biological replicates (i.e. cells from three different pigs), and batch 2 comprised two biological replicates. The estimates refer to the proportion of PAMs classified as infected (a & d), CD163 positive (b & e), and CD163 positive within the set of infected cells (c & f), after incubation for 0, 1, 2, 4, 6, 8 and 9 days, respectively, before PRRSV (infected) or mock-infection (control). Estimates refer to 18 h post (mock) infection. Predictions refer to refined model B with assumption AS1 (i.e. switching rates independent of CD163, σ_1,max = σ_2,max = σ_max)