Fig. 4
Phosphomutant functionality scoring based on metabolic profiles. The number of changing ions was scored between 0 and 3 according to the following scheme: 0 for no change; 1 for 1–4 changes; 2 for 5–19 changes; 3 for > 19 changes. The remaining three criteria were scored between 0 and 2 based to on the strongest p-value: 0 for p-value > 10−2; 1 for p-value < 10−2; 2 for p-value < 10−3. Mutants exhibiting a growth phenotype in the growth analysis are highlighted in blue