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Fig. 5 | BMC Systems Biology

Fig. 5

From: Untargeted metabolomics unravels functionalities of phosphorylation sites in Saccharomyces cerevisiae

Fig. 5

Metabolic profiles, HOG signaling activation and phenotype of Pbs2 phosphomutants. a Log2 fold changes of all ions corresponding to deprotonated metabolites of Hog OUT compared to Pbs2 IN in pyruvate/NH4 + media. b Log2 fold changes of all ions corresponding to deprotonated metabolites of Hog OUT compared to Pbs2 OUT in pyruvate/NH4 + media. c Time course Western blot of Hog1 activity in wild-type and Pbs2 phosphomutants, measured as phosphorylated Hog1 (P-Hog1) upon osmotic stress. Total Hog1 (t-Hog1) was measured as control. d Phenotype analysis upon osmotic stress and exposure to different temperatures. Spotting assay of Pbs2 phosphomutants and Hog1 OUT in synthetic complete (SC)-glucose, SC-glucose supplemented with 0.5 M NaCl, SC-glucose at 42 °C and SC-glucose at 16 °C. The assay was done three times, the results of one exemplary experiment are shown

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