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Table 3 Estimation of the apparent permeability coefficient of ammonium for IMZ351 (Δmep) into the plasma membrane

From: Membrane potential independent transport of NH3 in the absence of ammonium permeases in Saccharomyces cerevisiae

Strain

pHEC

pHvac

Cytosolic NH3 (μmol/LCyt)

Extracellular NH3 (μmol/LEC)

Estimated Cyt/EC ratio

Apparent permeability coefficient (m/h)

IMZ351

5.0a

4.2a

0.37

0.39

0.030

2.73a

6.0

4.5

1.31

1.47

0.283

0.37

7.0

4.5

0.90

3.16

0.902

0.03

  1. a In this particular case, a numerical solution to the system of algebraic equations that estimates P 1a (Additional file 1) is achieved only if the vacuolar pH was 4.2 and the vacuolar volume considered was 25% of the total cell volume
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BMC Systems Biology

ISSN: 1752-0509

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