Table 4 Overview of measured extracellular fluxes and N-content of IME169 (reference strain) and IMZ351 (Δmep) during N-limited aerobic chemostats in synthetic medium with glucose at a dilution rate of 0.05 h−1 at different extracellular pH
Strain | pHEC | μ | -qS | -qO2 | qCO2 | qEthanol | -qN | N-content | YXS | C/N consumption | qATP | qATP/-qN |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1/h | mmol/gCDW/h | mmol/gCDW/h | mmol/gCDW/h | mmol/gCDW/h | mmol/gCDW/h | mmol N/gCDW | gCDW/gGlc | C-mol/N-mol | mmol/gCDW/h | mol ATP/mol N | ||
IME169 | 5.0 | 0.053 ± 0.001 | 3.862 ± 0.050 | 1.643 ± 0.006 | 7.028 ± 0.015 | 4.601 ± 0.223 | 0.251 ± 0.001 | 4.70 ± 0.01 | 0.077 ± 0.001 | 92.3 ± 1.3 | 7.72 ± 0.22 | 30.8 ± 0.9 |
6.0 | 0.052 ± 0.001 | 3.398 ± 0.013 | 1.468 ± 0.004 | 6.157 ± 0.010 | 4.438 ± 0.055 | 0.223 ± 0.004 | 4.30 ± 0.08 | 0.085 ± 0.001 | 91.4 ± 1.7 | 7.23 ± 0.05 | 32.4 ± 0.6 | |
7.0 | 0.051 ± 0.001 | 2.953 ± 0.013 | 1.273 ± 0.007 | 5.218 ± 0.020 | 3.608 ± 0.038 | 0.208 ± 0.004 | 4.06 ± 0.08 | 0.096 ± 0.001 | 104.1 ± 2.3 | 6.03 ± 0.04 | 28.9 ± 0.6 | |
IMZ351 | 5.0 | 0.047 ± 0.001 | 3.485 ± 0.025 | 1.390 ± 0.005 | 6.620 ± 0.014 | 4.735 ± 0.039 | 0.190 ± 0.008 | 4.00 ± 0.17 | 0.081 ± 0.001 | 110.1 ± 4.7 | 7.38 ± 0.04 | 38.9 ± 1.6 |
6.0 | 0.047 ± 0.001 | 3.074 ± 0.017 | 1.223 ± 0.006 | 5.825 ± 0.028 | 4.404 ± 0.046 | 0.183 ± 0.003 | 3.91 ± 0.06 | 0.085 ± 0.001 | 100.8 ± 1.7 | 6.73 ± 0.05 | 36.7 ± 0.6 | |
7.0 | 0.048 ± 0.001 | 2.826 ± 0.031 | 1.239 ± 0.004 | 5.081 ± 0.009 | 3.639 ± 0.053 | 0.187 ± 0.003 | 3.88 ± 0.06 | 0.095 ± 0.001 | 90.7 ± 1.8 | 5.99 ± 0.05 | 32.0 ± 0.5 |