Table 3 Unannotated metabolic reactions in the T. erythraeum genome but included in the model based on homology to related organisms and/or to close gaps for biomass formation
Pathway | Proposed function | E.C. Number | Gene | Annotated function | Closest organism |
|---|---|---|---|---|---|
Newly/Improved Annotated | |||||
 Amino Acid Metabolism | L-alanine: glyoxylate aminotransferase | 2.6.1.44 | Tery_3167 | Serine: glyoxylate transaminase | Leptolyngbya sp. NIES 3755 |
L-serine: pyruvate aminotransferase | 2.6.1.44, 2.6.1.45, 2.6.1.51 | Tery_3167 | Serine: glyoxylate transaminase | Leptolyngbya sp. NIES 3755 | |
L-aspartase | 4.3.1.1 | Tery_1328 | Fumarase | Nitrosococcus oceani | |
L-arogenate: 2-oxoglutarate aminotransferase | 2.6.1.79 | Tery_0293 | L-aspartate aminotransferase | Pleurocapsa sp. PCC 7327 | |
L-threonine ammonium-lyase | 4.3.1.19 | Tery_4742 | Pyridoxal-5’-phosphate-dependent enzyme, beta subunit/cysteine synthase A | Zymomonas mobilis subsp. NRRL B-12526 | |
 Isoprenoid Synthesis | Tocopherol phytyltransferase | 2.5.1.117 | Tery_3881 | Homogentisate phytyltransferase | Nostoc sp. NIES-3756 |
 Pigment Metabolism | Chlorophyllide-a: NADP+ oxidoreductase | 1.1.5.31.3.1.75 | 18445Tery_3563 | NmrA-like | Amborella trichopoda |
 Secondary Carbon Metabolism | Citramalate synthase | 2.3.1.182 | Tery_2253 | 2-Isopropylmalate synthase | Dehalococcoides mccartyi VS |
 Sulfur Metabolism | O-succinylhomoserine thiol lyase | 2.5.1.48 | Tery_0352 | 8-Amino-7-oxononanoate synthase | Nostoc sp. PCC 7524 |
 Tricarboxylic Acid Cycle | Isocitrate lyase | 4.1.3.1 | Tery_4268 | 2,3-Dimethylmalate lyase/methylisocitrate lyase | Candidatus Nitrososphaera gargensis |
Assumed Promiscuous | |||||
 Amino Acid Metabolism | 4-Hydroxyglutamate transaminase | 2.6.1.23 | Tery_0293 | L-aspartate aminotransferase | T. erythraeum |
 Cofactor and Energy Carrier Metabolism | Dihydroneopterin P i dephosphorylase | 3.6.1.1 | Tery_1519 | Inorganic diphosphatase | |
Dihydroneopterin PPP i dephosphorylase | 3.6.1.1 | Tery_1519 | Inorganic diphosphatase | ||
 Lipid Metabolism/Secondary Carbon Metabolism | Glycoaldehyde dehydrogenase | 1.2.1.21 | Tery_2599 | Aldehyde dehydrogenase | |
 Nucleotide Metabolism | 3’-5’-Nucleotide phosphodiesterase: cAMP | 3.1.4.17 | Many | 3’-5’-Nucleotide phosphodiesterase: NMP | |
Missing (but Essential) Gene | |||||
 Cofactor and Energy Carrier Metabolism | (R)-Pantoate:NADP+2-oxidoreductase | 1.1.1.169 | None | None | None |