The use of genome-scale metabolic network reconstruction to predict fluxes and equilibrium composition of N-fixing versus C-fixing cells in a diazotrophic cyanobacterium, Trichodesmium erythraeum

Table 6 Constraints for each cell type in model simulations for FBA and FVA

Parameter	Diazotroph	Photoautotroph
Carbon Uptake (mmol C (g DW)^-1 h^-1)	0.927 (glycogen) [29]	0.927 (CO₂) [29]
Nitrogen Uptake (mmol N (g DW)^-1 h^-1)	Unlimited (N₂)	Unlimited (NH₄ ⁺)
Nitrogenase Flux (mmol (g DW)^-1 h^-1)	0.132	0
Maintenance Energy (mmol ATP (g DW)^-1 h^-1)	64.3	67.2
hν ^PSI	80	80 − hν ^PSII
hν ^PSII	0	80 − hν ^PSI
Growth Rate (h^-1)	0.0146 [29]	0.0146 [29]
Metabolite Output	NH₄ ⁺	Glycogen
Objective Function	Maximize biomass	Maximize biomass

ISSN: 1752-0509