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Fig. 2 | BMC Systems Biology

Fig. 2

From: Synthetic circuits that process multiple light and chemical signal inputs

Fig. 2

The design of the 3-input circuit. (a) Illustration of the conversion of input signals including Dox, cumate and light into graded output signals, which are promoter activities. The system consists of three inducible systems formed Boolean logic gates. (b) The scheme of the circuit. It can be divided into three layers. In the first layer, LightOn system controls the expression of mCherry-TCP fusion; cumate-switch system controls the expression of rtTAm (co-expressed with EYFP linked by 2A peptide). In the second layer, Dox or mCherry-TCP fusion protein can serve as an inducer to trigger the binding of rtTAm to the cognate TRE3G promoter. In the third layer, rtTAm serves as transcription activator to initiate the transcription of luciferase from TRE3G promoter

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