Fig. 3
From: Synthetic circuits that process multiple light and chemical signal inputs
TCP induces rtTAm. (a) Scheme of TCP induced rtTAm binding to TRE3G promoter. Constitutively expressed mCherry-TCP and rtTAm (both driven by CMV promoter) interact with each other, and then the complex bind to the tetO elements within TRE3G promoter (consist of 7 × tetO elements and a minimal CMV promoter). A GFP gene is placed downstream of TRE3G promoter. (b) mCherry-TCP induced the rtTAm-dependent expression of GFP. GFP-positive cells were observed in cells co-transfected with mCherry-TCP and rtTAm coding plasmids, but not in cells co-transfected with mCherry and rtTAm coding plasmids. The scale bar is 50 μm