Skip to main content
Fig. 5 | BMC Systems Biology

Fig. 5

From: Synthetic circuits that process multiple light and chemical signal inputs

Fig. 5

Characterization of the complete 3-input circuit. Microscopy images, showing the expression of mCherry-TCP (Cy3 filter) and EYFP (YFP filter) of cells treated with all eight combinations of three inducers, are presented beneath the corresponding values of luciferase activity. Scale bar in the last image is 100 μl. All the images were taken at the same magnification. The x-axis indicates the specific treatments. “+” represents illumination with blue LED (1.25 W m−2) for 24 h then incubated in the dark for another 24 h, treatment of 1 μg/ml of Dox, or treatment of 30 μg/ml of cumate, respectively. “-” represents no corresponding treatments. Expression of luciferase of the cells treated with various input signals combinations was examined. The y-axis shows luciferase activity. Data are presented as mean ± SD (n = 6)

Back to article page