Table 1 list of Proteins used in this study, functional annotation based on Genecards [http://www.genecards.org/], and dynamic profile classification. Down–decrease in time, Down-up decrease in first 12 h followed by increase, up–increase over time
From: Dynamic proteomics reveals bimodal protein dynamics of cancer cells in response to HSP90 inhibitor
Protein name | Description | Dynamics |
|---|---|---|
CKS2 | CDC28 protein kinase, down regulated by p53, G1/S phase transition | Up |
BAG1 | blocks apoptosis, enhances anti-apoptotic effects of BCL2, Inhibits chaperone activity | |
VPS26A | retromer complex, retrieve lysosomal enzyme receptors from endosomes to the Golgi | |
H2AFV | H2A histone protein, nucleosome assembly | Down-up |
UBE2K | ubiquitin enzyme E2, p53 degradation, NRF2 Oxidative Stress Response | |
CACYBP | ubiquitin degradation, beta-catenin degradation, positive regulation of DNA replication | |
RAB11A | RAS oncogene, membrane delivery during cytokinesis, protein transport, endocytosis | |
TXN | Upregulate JUN & NFkB, apoptosis | |
STMN1 | destabilizing microtubules, mitotic spindle organization, activated by ERK | |
STK24 | serine/threonine kinase, apoptosis, upstream (MAPK) cascade | |
RPS3A | ribosomal protein, translation, induction of apoptosis (BCL2 regulation) | |
TXNRD1 | response to oxidative stress, induces actin &tubulin polymerization, cell death | |
FSCN1 | actin-bundling protein, cell motility & migration, cell proliferation | |
RBX1 | E3 ubiquitin ligase, cell cycle progression, DNA repair | |
SUMO1 | ubiquitin-like, DNA repair, upregulate P53, nuclear transport, apoptosis, protein stability | |
PAK2 | p21 activated kinase, cytoskeleton reorganization, anti apoptosis | |
TBCA | tubulin folding, stabilizing beta-tubulin | |
SPCS1 | proteolysis, metabolic process, removes signal peptides from proteins in the ER | |
PRKAR2A | kinase, cAMP-dependent, lipid&glucose metabolism, signaling of GPCR, anti apoptosis | |
CALM2 | regulates the centrosome cycle and cytokinesis | |
MAPK1 | Proliferation, transformation- also known as ERK2, kinase | |
IL3RA | interleukin 3 receptor, Immune response IL-3, Apoptosis, Jak-STAT signaling pathway | |
PLEC1 | intermediate filaments with MT & microfilaments, apoptosis, cell junction assembly | |
PTPN11 | Phosphatase, an upstream activator of Src & ras, Proliferation, transformation | |
BAG2 | BCL2-associated, Inhibits chaperones, apoptosis | |
PCMTD2 | protein modification, protein-L-isoaspartate(D-aspartate)O-methyltransferase domain | |
VIL2 | ezrin, intermediate between the PM and actin cytoskeleton, activate MET with CD44 | |
APAF1 | initiates apoptosis | |
MAP2K2 | kinase, Activates the ERK1 and ERK2, Proliferation, transformation | |
RFC1 | replication & repair factor, subunit DNA polymerase, S phase of mitotic cell cycle | |
TJP1 | tight junction protein, apoptosis | |
EEF2 | elongation factor, protein synthesis | |
DDX5 | RNA helicases, splicing, cellular growth and division, P53 TF | |
PSMB4 | proteasome, cell cycle checkpoint | |
MAPKAP1 | mTORC2 Subunit, regulates cell growth & survival, regulate the actin cytoskeleton | |
ENO1 | Enolase, glycolysis, growth control, hypoxia tolerance, receptor, down regulate myc | |
PBX3 | Transcriptional activator that binds the sequence 5-ATCAATCAA-3 | |
RPL11 | ribosomal protein L11, rRNA processing, translation | |
LMNA | nuclear membrane fibrous, apoptosis, During mitosis disassembled, nuclear stability | |
CDKN3 | cell cycle arrest | |
RPS3 | ribosomal protein, translation, induction of apoptosis, repair UV-induced DNA damage | |
FBL | snRNP, pre-r/tRNA processing, physical interaction with DDX5, coexpress with NCL | |
AKAP8L | nuclear envelope breakdown & chromatin condensation. initiation of DNA replication | |
PSMC4 | proteasome cell cycle checkpoint | Down |
HDAC2 | histone deacetylase, cell growth arrest, differentiation and death | |
PSMB7 | proteasome cell cycle checkpoint | |
PDCD5 | induction of apoptosis | |
RRAS | Regulates the organization of the actin cytoskeleton, RAS related, activates RAF | |
DNMT1 | CpG methylation, activated by JUN, STAT3, involved in P53 AKT paths | |
K-alpha-1 | tubulin, alpha 1b | |
HMGA2 | negative transcription regulation, chromatin organization, G1/S and G2/M transitions | |
ARL3 | cytokinesis and cilia signaling, microtubule binding, intracellular protein transport | |
VCL | (F-actin)-binding protein, cell-matrix & cell-cell adhesion, cell morphology, locomotion | |
CD44 | MET activation, wound healing, inflammatory response, cell adhesion | |
PSMA1 | proteasome, cell cycle checkpoint, Immune response | |
PGK1 | phosphoglycerate kinase, glycolytic enzyme | |
ILF2 | immune response | |
RPL22 | ribosomal protein, component of the 60S subunit | |
PPP1R2 | carbohydrate metabolic process, phosphatase | |
PKN1 | JUN kinase activity, regulation of the actin cytoskeleton, TF | |
HMGA1 | regulation of transcription | |
PTTG1 | protein import into nucleus | |
NPM1 | chaperoning, histone assembly, cell proliferation, regulation of p53/TP53, ARF | |
NASP | transporting histones into the nucleus, cell proliferation | |
PFDN5 | chaperone | |
HAT1 | internal protein A.A. acetylation, DNA packaging, HMGB1 signaling, DNA repair | |
STK4 | pro apoptosis regulator | |
RTN4 | pro apoptosis regulator | |
PAWR | PRKC, pro apoptosis regulator | |
SLBP | histone mRNA 3-end processing, mRNA export from nucleus | |
CFLAR | CASP8 pro apoptosis gene | |
YT521 | RNA splicing (May be part of a signaling and alternative splicing) | |
ANXA2 | Calcium-regulated membrane-binding protein, signal transduction | |
DNCH1 | Microtubule molecular motors-cytoplasmatic. |