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Table 5 Comparison experimental and simulated yields of wild type and mutant strains of Clostridium sp. IBUN 158B cultured in glycerol [73]

From: Clostridium butyricum maximizes growth while minimizing enzyme usage and ATP production: metabolic flux distribution of a strain cultured in glycerol

Strain

Experimental yields

Simulated yields (FVA range)

 

YX/S a

YPDO/S

YX/S a

YPDO/S

Wild strain

100.0 ± 8%

0.538 ± 0.047

100%

0.588 (0.479–0.656)

ΔhydA-420 s

46.7 ± 9%

0.465 ± 0.031

75.8%

0.610 (0.478–0.721)

ΔldhA-508 s

61.6 ± 3%

0.579 ± 0.021

98.9%

0.595 (0.489–0.659)

Δhbd-414 s

Not available b

95.2%

0.572 (0.448–0.656)

  1. aBiomass Yields were normalized based on the wild type strain value
  2. bData not measured experimentally by Montoya [73]
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BMC Systems Biology

ISSN: 1752-0509

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