Remodeling adipose tissue through in silico modulation of fat storage for the prevention of type 2 diabetes

BMC Systems Biology

Table 1 List of tests which failed and the genes added to correct them

Failed validation^a	Gene(s) added to correct
UDP-N-acetyl D-galactosamine de novo synthesis	GNPNAT1, GALE
CMP-N-acetylneuraminate de novo synthesis	GNPNAT1
N-Acetylglucosamine de novo synthesis	GNPNAT1, RENBP
Galactose degradation	GALE
UDP-galactose de novo synthesis	GALE
Lactosylceramide de novo synthesis	GALE
Cysteine de novo synthesis	CBS
Methionine degradation	CBS
Homocysteine degradation	CBS
CoA de novo synthesis	CBS, SLC5A6
GSH de novo synthesis	CBS
Histidine degradation	FTCD, UROC1
Phenylalanine degradation	HGD
Tyrosine degradation	HGD
Tryptophan degradation	HAAO, AFMID, ACMSD, SLC25A21
Heme de novo synthesis	SLC46A1

List of validation tests which wrongly failed in the metabolic network and that were fixed by the addition of identified genes and associated reactions
GNPNAT1 Glucosamie-Phosphate N-Acetyltransferase 1, GALE UDP-Galactose-4-Epimerase, RENBP Renin Binding Protein, CBS Cystathionine-Beta-Synthase, SLC5A6 Solute Carrier Family 5 Member 6, FTCD Formimidoyltransferase Cyclodeaminase, UROC1 Urocanate Hydratase 1, HGD Homogentisate 1,2-Dioxygenase, HAAO 3-Hydroxyanthranilate 3,4-Dioxygenase, AFMID Arylformamidase, ACMSD Aminocarboxymurconate Semialdehyde Decarboxylase, SLC25A21 Solute Carrier Family 25 Member 21, SLC46A1 Solute Carrier Family 46 Member 1
^a A list of all validations is available in Additional file 3: Table S2

ISSN: 1752-0509