Fig. 4
From: Cooperative binding mitigates the high-dose hook effect
Reduced hook effect in cooperative (wt) calmodulin. This figure shows the results of simulations on wildtype calmodulin (which is allosterically regulated, in black) compared to a non-cooperative in silico mutant (R state only, in red). The plot of fully bound calmodulin as a function of initial calmodulin concentration shows a prozone effect in both cases, but it is more pronounced in the non-cooperative version