Fig. 5

Molecular regulatory system of cell cycle (G0 → G1/G1 → S). Since the HSCs in which Maff and Egr3 are highly expressed (i.e. functional) are mainly G0-phase cells (over 90%), it implicates that if Maff and Egr3 have influence(s) on cell cycle, they must exert influence(s) in G0 → G1/G1 → S in the first place. Therefore, as a preliminary attempt, we summarized all key regulators in G0 → G1/G1 → S (including two cyclins, five CKIs, Rb, E2F and Akt) and examined all possible ways that Maff and Egr3 (highlighted in red) act on the molecular network that result in consistent phenomena with experimental observations. Symbols: “−⊣” - inhibition; “―→” - activation; solid lines - transcriptional regulations; dashed lines - biochemical interactions; red lines: computionally inferred molecular regulatory relationships mediated by Maff and Egr3