Fig. 3

Expression along a synthetic compensatory path to e251. a-e For each sequence, the binding structure structure is shown (left). Height of bars is proportional to LLR of binding for each motif. A subset of motifs are shown. Binding sites for all factors considered in this work are included in Additional file 1: Figures S5-S15. We show FISH for lacZ driven by each of the sequences (center). We also show the quantitative level of mRNA driven by each enhancer along a 10% DV stripe from 35.5% to 92.5% embryo length (right). Data represents an average of n images, where the value of n is indicated. a MSE2. b e24 (24 LE removed from MSE2). c e36 (36 LE removed from MSE2). e e48 (48 LE removed from MSE2). e e60 (60 LE removed from MSE2). f The number of Bcd, Hb, Kr, and Gt binding sites in MSE2 that are maintained in each of the specified synthetic enhancers. The percent of binding sites (LLR>0) that are maintained is given above each bar. g The number of Bcd, Hb, Kr, and Gt binding sites (LLR>0) that are gained with respect to MSE2 are shown for each synthetic enhancer. h The number Bcd, Hb, Kr, and Gt binding sites (LLR>0) that are lost with respect to MSE2 are shown for each factor. blackThe percent of sites lost is given above each bar