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Fig. 5 | BMC Systems Biology

Fig. 5

From: Synthetic enhancer design by in silico compensatory evolution reveals flexibility and constraint in cis-regulation

Fig. 5

A 319bp synthetic enhancer drives stripe 2 at levels more than 10 times greater than MSE2. a The binding structure of a sequence designed, with model feedback, to drive expression of a stripe 2 pattern is shown. Height of bars is proportional to the LLR. Most sites represent consensus motifs for each factor. Binding sites for all factors considered in this work are included in Additional file 1: Figures S5-S15. b The predicted root mean squared differences from MSE2 expression along a series of 272 edits that would transform MSE2 into the sequence in panel A. 10 sequences (open circles) that were tested in vivo are shown. c The binding structure of MSE2 (left). FISH for lacZ driven by MSE2 (center). Quantitative levels driven by MSE2 (right). The number of averaged images, n, is indicated. d The binding structure of s100 (left). FISH for lacZ driven by s100 (center). Quantitative levels (right) driven s100, a sequence 100 edits from MSE2. This sequence drives expression of levels slightly less than MSE2. The number of averaged images, n, is indicated. e The binding structure of s250 (left). FISH for lacZ driven by s250 (center). Quantitative levels (right) driven by a s250, a 319 bp sequence 250 edits from MSE2. This sequence drives expression at levels more than 10 times greater than MSE2. The number of averaged images, n, is indicated

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