Fig. 4

Effects of Isorhamnetin and Kaempferide on melanin production in B16F10 melanoma cells. Appearance of the recovered cell pellets in test tubes by Isorhamnetin (a). The intracellular melanin contents in Isorhamnetin-treated B16F10 melanoma cells for 24 h (b). Extracellular melanin contents in Isorhamnetin-treated B16F10 melanoma cells for 24 h (c). Appearance of the recovered cell pellets in test tubes by Kaempferide (d). Intracellular melanin contents in Kaempferide-treated B16F10 melanoma cells for 24 h (e). Extracellular melanin contents in Kaempferide-treated B16F10 melanoma cells for 24 h (f). Data shown represent the means ± standard error of at least 3 independent experiments performed in triplicate. ^* P < 0.05 vs. the control, ^# P < 0.05 vs. 8-MOP (100 μM)