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Table 1 Promoters and terminators of the P. pastoris GoldenPiCS toolbox

From: GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

Promoter/Terminator Short Name Gene name ORF length [bp] μA mean exp. G μA mean exp. X μA mean exp. D μA mean exp. M rel. eGFP [%] G rel. eGFP [%] X rel. eGFP [%] D rel. eGFP [%] M
P GAP TDH3 glyceraldehyde-3-phosphate dehydrogenase PP7435_Chr2–0858 496 175,734 153,293 190,970 89,048 100 100 100 100
P GPM1 GPM1 phosphoglycerate mutase PP7435_Chr3–0360 404 118,686 98,941 165,884 52,844 25 13 26 11
P RPP1B RPP1B ribosomal protein P1 beta PP7435_Chr4–0276 999 168,241 88,081 147,457 134,220 22 14 23 13
P PDC1 PDC1 pyruvate decarboxylase 1 PP7435_Chr3–1042 989 95,047 34,921 188,242 24,093 19 17 23 16
P POR1 POR1 mitochondrial porin PP7435_Chr2–0411 704 94,576 173,321 58,699 118,862 46 28 19 51
P MDH3 MDH3 peroxisomal malate dehydrogenase PP7435_Chr4–0136 979 26,497 206,905 12,554 87,661 76 83 17 110
P ADH2 ADH2 alcohol dehydrogenase 2 PP7435_Chr2–0821 820 117,961 165,203 140,678 61,540 33 47 16 54
P FBA1–1 FBA1–1 fructose 1,6-bisphosphate aldolase PP7435_Chr1–0374 970 152,990 127,354 213,816 57,961 20 11 14 12
P SHB17 SHB17 sedoheptulose bisphosphatase PP7435_Chr2–0185 995 24,473 43,866 35,852 167,426 0 0 0 53
P TEF2 TEF2 translational elongation factor EF-1 alpha PP7435_Chr1–1535 572 184,546 96,226 180,287 161,445    53  
P RPL2A RPL2A ribosomal 60S subunit protein L2A PP7435_Chr4–0909 949 184,025 96,715 164,082 143,041    10  
P LAT1 LAT1 dihydrolipoamide acetyltransferase PP7435_Chr1–0349 1006 24,576 22,867 31,661 16,960    6  
P PFK300 PFK300 Gamma subunit of 6-phosphofructokinase PP7435_Chr4–0686 229 20,845 18,516 21,844 15,474    2  
P GUT1 GUT1 glycerol kinase PP7435_Chr4–0173 1305 31,172 88,114 1324 12,564 44   1  
P THI11 THI11 involved in synthesis of thiamine precursor PP7435_Chr4–0952 1005 77,578 101,948 45,756 120,542    36  
P DAS2 DAS2 dihydroxyacetone synthase 2 PP7435_Chr3–0350 1005 1316 32,401 1268 186,477    0 160
P AOX1 AOX1 alcohol oxidase 1 PP7435_Chr4–0130 928 5301 159,461 4487 188,502    0 105
P DAS1 DAS1 dihydroxyacetone synthase 1 PP7435_Chr3–0352 554 2518 45,547 2200 239,261    0 99
P FDH1 FDH1 formate dehydrogenase PP7435_Chr3–0238 1032 3126 215,618 2418 179,036    0 83
P ALD4 ALD4 mitochondrial aldehyde dehydrogenase PP7435_Chr2–0787 869 21,239 109,237 9330 52,434    0 18
ScCYC1tt YJR048W cytochrome C isoform 1 Sc: YJR048W 285     100 100 100
TDH3tt TDH3 glyceraldehyde-3-phosphate dehydrogenase PP7435_Chr2–0858 210 175,734 153,293 190,970 89,048   139 128 126
RPS2tt RPS2 protein component of the 40S ribosome PP7435_Chr1–1396 476 161,620 77,215 151,802 135,407   136 129 128
RPP1Btt RPP1B ribosomal protein P1 beta PP7435_Chr4–0276 507 168,241 88,081 147,457 134,220   120 117 113
RPS17Btt RPS17B ribosomal protein 51 PP7435_Chr2–0491 495 122,828 89,591 125,205 117,162   124 122 118
chr4_0883tt chr4_0883 not conserved hypothetical protein PP7435_Chr4–0069 483 127,795 93,922 95,185 113,403   88 99 84
IDP1tt IDP1 mitochondrial NADP-specific isocitrate dehydrogenase PP7435_Chr1–0546 503 11,349 8309 19,236 10,932   126 123 118
RPS25Att RPS25A protein component of the 40S ribosome PP7435_Chr2–0346 493 146,211 100,973 140,035 126,110   140 126 123
RPS3tt RPS3 protein component of the 40S ribosome PP7435_Chr1–0118 174 143,928 59,278 143,257 121,372   137 125 123
RPL2Att RPL2A ribosomal 60S subunit protein L2A PP7435_Chr4–0909 504 184,025 96,715 164,082 143,041   102 102 93
  1. Mean expression from microarray (μA) data were extracted from [26]. The relative eGFP expression is related to expression under control of PGAP in the respective condition
  2. G 2% glycerol, X glucose feed bead (limited glucose), D 2% glucose, M methanol
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