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Table 1 Promoters and terminators of the P. pastoris GoldenPiCS toolbox

From: GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

Promoter/Terminator

Short Name

Gene name

ORF

length [bp]

μA mean exp. G

μA mean exp. X

μA mean exp. D

μA mean exp. M

rel. eGFP [%] G

rel. eGFP [%] X

rel. eGFP [%] D

rel. eGFP [%] M

P GAP

TDH3

glyceraldehyde-3-phosphate dehydrogenase

PP7435_Chr2–0858

496

175,734

153,293

190,970

89,048

100

100

100

100

P GPM1

GPM1

phosphoglycerate mutase

PP7435_Chr3–0360

404

118,686

98,941

165,884

52,844

25

13

26

11

P RPP1B

RPP1B

ribosomal protein P1 beta

PP7435_Chr4–0276

999

168,241

88,081

147,457

134,220

22

14

23

13

P PDC1

PDC1

pyruvate decarboxylase 1

PP7435_Chr3–1042

989

95,047

34,921

188,242

24,093

19

17

23

16

P POR1

POR1

mitochondrial porin

PP7435_Chr2–0411

704

94,576

173,321

58,699

118,862

46

28

19

51

P MDH3

MDH3

peroxisomal malate dehydrogenase

PP7435_Chr4–0136

979

26,497

206,905

12,554

87,661

76

83

17

110

P ADH2

ADH2

alcohol dehydrogenase 2

PP7435_Chr2–0821

820

117,961

165,203

140,678

61,540

33

47

16

54

P FBA1–1

FBA1–1

fructose 1,6-bisphosphate aldolase

PP7435_Chr1–0374

970

152,990

127,354

213,816

57,961

20

11

14

12

P SHB17

SHB17

sedoheptulose bisphosphatase

PP7435_Chr2–0185

995

24,473

43,866

35,852

167,426

0

0

0

53

P TEF2

TEF2

translational elongation factor EF-1 alpha

PP7435_Chr1–1535

572

184,546

96,226

180,287

161,445

  

53

 

P RPL2A

RPL2A

ribosomal 60S subunit protein L2A

PP7435_Chr4–0909

949

184,025

96,715

164,082

143,041

  

10

 

P LAT1

LAT1

dihydrolipoamide acetyltransferase

PP7435_Chr1–0349

1006

24,576

22,867

31,661

16,960

  

6

 

P PFK300

PFK300

Gamma subunit of 6-phosphofructokinase

PP7435_Chr4–0686

229

20,845

18,516

21,844

15,474

  

2

 

P GUT1

GUT1

glycerol kinase

PP7435_Chr4–0173

1305

31,172

88,114

1324

12,564

44

 

1

 

P THI11

THI11

involved in synthesis of thiamine precursor

PP7435_Chr4–0952

1005

77,578

101,948

45,756

120,542

  

36

 

P DAS2

DAS2

dihydroxyacetone synthase 2

PP7435_Chr3–0350

1005

1316

32,401

1268

186,477

  

0

160

P AOX1

AOX1

alcohol oxidase 1

PP7435_Chr4–0130

928

5301

159,461

4487

188,502

  

0

105

P DAS1

DAS1

dihydroxyacetone synthase 1

PP7435_Chr3–0352

554

2518

45,547

2200

239,261

  

0

99

P FDH1

FDH1

formate dehydrogenase

PP7435_Chr3–0238

1032

3126

215,618

2418

179,036

  

0

83

P ALD4

ALD4

mitochondrial aldehyde dehydrogenase

PP7435_Chr2–0787

869

21,239

109,237

9330

52,434

  

0

18

ScCYC1tt

YJR048W

cytochrome C isoform 1

Sc: YJR048W

285

   

100

100

100

TDH3tt

TDH3

glyceraldehyde-3-phosphate dehydrogenase

PP7435_Chr2–0858

210

175,734

153,293

190,970

89,048

 

139

128

126

RPS2tt

RPS2

protein component of the 40S ribosome

PP7435_Chr1–1396

476

161,620

77,215

151,802

135,407

 

136

129

128

RPP1Btt

RPP1B

ribosomal protein P1 beta

PP7435_Chr4–0276

507

168,241

88,081

147,457

134,220

 

120

117

113

RPS17Btt

RPS17B

ribosomal protein 51

PP7435_Chr2–0491

495

122,828

89,591

125,205

117,162

 

124

122

118

chr4_0883tt

chr4_0883

not conserved hypothetical protein

PP7435_Chr4–0069

483

127,795

93,922

95,185

113,403

 

88

99

84

IDP1tt

IDP1

mitochondrial NADP-specific isocitrate dehydrogenase

PP7435_Chr1–0546

503

11,349

8309

19,236

10,932

 

126

123

118

RPS25Att

RPS25A

protein component of the 40S ribosome

PP7435_Chr2–0346

493

146,211

100,973

140,035

126,110

 

140

126

123

RPS3tt

RPS3

protein component of the 40S ribosome

PP7435_Chr1–0118

174

143,928

59,278

143,257

121,372

 

137

125

123

RPL2Att

RPL2A

ribosomal 60S subunit protein L2A

PP7435_Chr4–0909

504

184,025

96,715

164,082

143,041

 

102

102

93

  1. Mean expression from microarray (μA) data were extracted from [26]. The relative eGFP expression is related to expression under control of PGAP in the respective condition
  2. G 2% glycerol, X glucose feed bead (limited glucose), D 2% glucose, M methanol