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BMC Systems Biology

Table 2 Biomass and optical density of the constructed gene deletion strains

Strain	Growth rate	Final biomass
(without plasmid)	μ _max (h ⁻¹)	(OD₆₀₀)
C. glutamicum DelAro⁴- 4c l _Pc	0.34±0.01	55.2±1.7
C. glutamicum DelAro⁴- 4c l _Pc Δ d d h	0.35±0.01	54.3±0.4
C. glutamicum DelAro⁴- 4c l _Pc Δ s d h C A B	0.27±0.02	28.1±0.7
C. glutamicum DelAro⁴- 4c l _Pc Δ d d h Δ s d h C A B	0.27±0.01	29.2±0.1

The maximal growth rate and the final biomass were analyzed for the constructed gene deletion strains not harboring the plasmid pMKEx2_chs _Ph_chs _Ph. The cultivation was performed in absence of plasmid and inducer of plasmid-borne gene expression to avoid any overlapping effects on growth behavior either resulting from the deleted genes or from differences in obtained naringenin titers/ expression of heterologous genes. Data represent average values and standard deviation obtained from three biological replicates

ISSN: 1752-0509