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Table 2 Biomass and optical density of the constructed gene deletion strains

From: OptPipe - a pipeline for optimizing metabolic engineering targets

Strain Growth rate Final biomass
(without plasmid) μ max (h −1) (OD600)
C. glutamicum DelAro4- 4c l Pc 0.34±0.01 55.2±1.7
C. glutamicum DelAro4- 4c l Pc Δ d d h 0.35±0.01 54.3±0.4
C. glutamicum DelAro4- 4c l Pc Δ s d h C A B 0.27±0.02 28.1±0.7
C. glutamicum DelAro4- 4c l Pc Δ d d h Δ s d h C A B 0.27±0.01 29.2±0.1
  1. The maximal growth rate and the final biomass were analyzed for the constructed gene deletion strains not harboring the plasmid pMKEx2_chs Ph _chs Ph . The cultivation was performed in absence of plasmid and inducer of plasmid-borne gene expression to avoid any overlapping effects on growth behavior either resulting from the deleted genes or from differences in obtained naringenin titers/ expression of heterologous genes. Data represent average values and standard deviation obtained from three biological replicates