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Fig. 1 | BMC Systems Biology

Fig. 1

From: Short linear motifs in intrinsically disordered regions modulate HOG signaling capacity

Fig. 1

Schematic diagram of the Sho1-dependant branch of the yeast high osmolarity glycerol (HOG) pathway as a model system for analysis of short linear motif function in signal transduction. Upon exposure to high osmolarity, the SH3 domain of the membrane-bound osmosensor Sho1 binds to PXXP motif in the MAPK kinase (MAPKK) Pbs2, initiating a phosphorylation cascade (grey arrows) that culminates in phosphorylation of the MAPK Hog1. Activated Hog1-P then accumulates inside the nucleus and initiates a complex transcriptional response in order to re-establish homeostasis within the cell. A Hog1-responsive promoter driving yeGFP reporter expression was used to quantify pathway activity for a series of deletions in predicted short linear motifs in pathway components – deleted residues shown in ovals for each module

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