Fig. 5

Comparison of chromatin features at PcTF-activated and non-activated genes in MCF7. a Box plots show expression levels (center line, median; left and right boxes, 25th and 75th percentiles; left and right whiskers, minimum and maximum) in untreated and PcTF-treated cells (24, 48, and 72 h) for each of the following gene subsets: PcTF-upreg., 125 genes that are upregulated (FC ≥ 2, p ≤ 0.05) in MCF7-expressing cells at one or more time points; Low BC, 45 H3K27me3-enriched genes that are repressed (FPKM < 2) in all three cancer cell lines (see Fig. 2c); Low MCF7, 50 genes that are repressed (FPKM < 2) in MCF7. b TSS plots show ChIP signals of silencing-associated (H3K27me3, H3K9me3) and activation-associated (H3K27ac, H3K4me3) histone modifications, as well as RNA Polymerase II. Genes within the top 20% of mean values for H3K27me3-enrichment (within 10 kb) are highlighted (blue box)