Fig. 5

Missing data imputation benefits to recover the trajectories of the primary somatosensory cortex and the hippocampal CA1 region single-cell RNA-seq data. The trajectories include seven cell types, such as astrocytes_ependymal in orange (n = 224), interneurons in chartreuse (n = 290), oligodendrocytes in aqua (n = 820), pyramidal SS in pink (n = 399), endothelial−mural in khaki (n = 235), microglia in green (n = 98) and pyramidal CA1 in purple (n = 939). a The single-cell RNA-seq expression trajectory analysis on the mouse brain cells without data imputation. b The trajectory analysis on the mouse brain cells using the method of mean-smooth neighbor cells on the trajectory. c The trajectories analysis on the mouse brain cells using MISC method to impute CML data