Fig. 1

Untargeted metabolomics identifies 215 metabolites in four brain regions of an adult mouse. From a proprietary library of Metabolon, Inc. containing > 2000 compounds, 215 metabolites were identified in the four brain regions: olfactory bulb, frontal cortex, hippocampus and cerebellum (N = 6 per region). a. Heat map represents the relative concentration of each metabolite organized by its respective metabolic category and brain region. Relative concentration of the metabolites was normalized by using studentized residual method. Color gradient represents the Z score distribution of each metabolite across all four regions, each containing six biological samples. Within-group variance is marginal. b. Eight major types of metabolites were identified. Shaded regions indicate their respective metabolic category and the number of metabolites in shown in parentheses. OB, olfactory bulb; FCX, frontal cortex; HC, hippocampus; CB, cerebellum