Table 2 Flask 1 population mutations

Δ thrA

4

0.58

metK

N226S

-

2

0.46

metK

G235A

-

2

0.23

metJ

V46E

*Reduce MetJ repression¹

1

0.79

metJ/metB

Intergenic (-211/-66)

*Reduce MetJ repression¹

3

-

117 genes

130 kbp, 1.2X GDA

Increase metL expression

[rrlA-rrfB]

Δ ptsI

4, 5

1.0, 0.82

ydhF

G65S

-

(1, 4,

(1.0, 1.0,

metK/galP

Intergenic (+328/-96)

*Reduce GalR repression²

5, 6)

0.36, 0.39)

5

0.25

metK/galP

Intergenic (+333/-91)

Reduce GalR repression²

3

1.0

metK/galP

Intergenic (+334/-90)

Reduce GalR repression²

6

0.57

metK/galP

Intergenic (+339/-85)

Reduce GalR repression²

3

1.0

crp

T141P

Global regulatory effects³

5

0.70

crp

G142S

Global regulatory effects³

6

0.59

crp

G142D

Global regulatory effects³

4

1.0

crp

R143H

Global regulatory effects³

1

1.0

crp

A145V

Global regulatory effects³

6

0.43

crp

I187T

Global regulatory effects³

7

1.0

96 genes

99 kbp, 2X GDA

Affect cyaA expression

[rrsC-rrlA]

Δ serB

1

0.67

hisL/hisG

Intergenic (+41/-105)

*Increase his operon expression⁴

5

0.43

yhaC/garK

Intergenic (+653/+370)

-

1

0.27

yhaC/garK

Intergenic (+883/+140)

-

6

0.88

hisR

His tRNA (5/77 bp)

Increase his operon expression⁴

2

0.82

hisR

His tRNA (48/77 bp)

Increase his operon expression⁴

4

0.92

hisR

His tRNA (67/77 bp)

*Increase his operon expression⁴

7

0.71

hisR

His tRNA (72/77 bp)

Increase his operon expression⁴

Δ proA

2

1.0

proB

1 bp Del

-

1

0.77

argD

G282D

Reduce ArgD activity

2

0.56

argD

Del (772-774/1221 bp)

Reduce ArgD activity

4

0.43

argD

Q154*

Reduce ArgD activity

3

0.34

argD

G49R

Reduce ArgD activity

Δ proB

6, 7

0.83, 0.72

glnA

F463L

Reduce GlnA activity

5

0.86

glnA

D187E

Reduce GlnA activity

4

0.45

glnA

G179C

Reduce GlnA activity

4

0.23

glnA

H172R

Reduce GlnA activity

1

1.0

glnA

G171S

Reduce GlnA activity

2

0.86

glnA

E156D

Reduce GlnA activity

3

1.0

glnA

S148F

Reduce GlnA activity

Δ carA

7

0.27

carA/ carB

Intergenic (+2/-16)

Increase carB expression

5

0.86

carB

L11L

-

3, 7

0.31, 0.81

rpoS

E96*

-

3, 7

1.0

508 genes

520 kbp, 2X GDA

Increase carB expression

[insD6-insD1]

Δ metC

4

0.55

malI

10bp Dup (227/1029 bp)

Reduce MalI activity⁵

2

0.32

malI

Q55*

Reduce MalI activity⁵

3

0.55

malX

Q529Q

-

Δ metL

2, 4

0.72, 0.38

rpoS

L317R

-

4

1.0

metB

1 bp Del (1144/1161 bp)

-

Δ cysK

1

1.0

2,062 genes

2.1 Mbp, 2X GDA

Increase cysM expression

[insL3-insL1]

3

0.21

sspA/rpsI

Intergenic (-10/+385)

-