Fig. 1

Overview of the process of including SLIME reactions and new lipid pseudo-reactions for a hypothetical model of three lipid classes and two types of acyl chain. The active fluxes after simulating the models are highlighted in light blue, showing that a GEM with a restrictive approach would use the same acyl chain composition for all lipid classes (left upper corner), a GEM with a permissive approach would always choose the cheapest species from each lipid class (left lower corner), and a GEM with SLIME reactions would satisfy both the lipid class and the acyl chain distribution, but choosing freely which specific lipid species to produce for this goal (right side)