Table 2 Nucleotide sequences of primers used for quantitative real-time PCR
From: Effects of unpaired 1 gene overexpression on the lifespan of Drosophila melanogaster
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product length (in bp) | Expected efficiency |
|---|---|---|---|---|
upd1 | agacagccgtcaaccagac | gcttcaaacgcttgttcatc | 141 | > 2 |
domeless | tcgctacatgacaacgtgaccgat | acgcacgctaatctcgtacttggt | 132 | > 2 |
Socs36E | gggcaaacagaacccagaaaccaa | tccgagctgcattccaataggtga | 189 | > 2 |
βTub56D | gcaactccactgccatcc | cctgctcctcctcgaact | 216 | > 2 |