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Fig. 2 | BMC Systems Biology

Fig. 2

From: LSM-W2: laser scanning microscopy worker for wheat leaf surface morphology

Fig. 2

Defining parameters of the epidermal jigsaw-puzzle cell pattern for mature maize leaf. a 2D image of the virtual cut reflecting the cellular structure of the epidermal layer with trichomes. b Heat map of the “leaf surface mask” with different colors corresponding to different values of z-slice number. The “leaf surface mask” was corrected in order to remove trichomes from the image A. c 2D image of the virtual cut reflecting the cellular structure of the epidermal layer without trichomes. d Watershed cell segmentation with manual correction for the maize leaf fragment. e Heat map marking of cells based on their “lobeyness”. Scale bars for A-E: 100 μm. f Scatter plot of cells’ area vs. cells’ “lobeyness” with Canberra distance function for three clusters: pavement cells (green), stomata (blue), and trichomes and other small cells (orange). The maize leaf fragment containing 1325 cells was used for the analysis. g Explanation of the term “lobeyness”, which is the ratio of the cell’s perimeter (orange) to the cell’s convex hull perimeter (black)

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