Figure 3
From: Annotating novel genes by integrating synthetic lethals and genomic information
Experimental validation of genes predicted to be involved in spindle migration. A) The she1 Δ, uba4 Δ and YHR127WΔ-strains show perturbed Kar9 localization. gpd1 Δ does not show a phenotypic effect. Wild type and tvp38 Δ are shown as positive and negative controls, respectively. The differences in proportions of defective cells are compared to the wild type based on the binomial distribution. This results in p-values that are significant for she1 Δ and uba4 Δ (p-values 0.0008% and 0.28% respectively), marginally significant for YHR127WΔ (p = 7.4%) and not significant for gpd1 Δ (p = 70%). B) Some she1 Δ cells have broken or bent anaphase spindles suggesting compromised spindle integrity.