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Table 6 Fluxes distribution for acetate and acetate + propionate feeding between mixed microbial cultures and Cupriavidus necator

From: Metabolic modelling of polyhydroxyalkanoate copolymers production by mixed microbial cultures

Feeding

Acetate

Acetate + Propionate

Culture enrichment

Acetate

Propionate

 

Acetate

Propionate

 

Cultures

Mixed microbial cultures (This work)

Cupriavidus necator[37]

Mixed microbial cultures (This work)

Cupriavidus necator[18]

 

Substrate uptake rate (C-mol/(C-mol.h))

VFA uptake rate

0.73

0.21

≈ 0.441

0.67

0.45

≈ 0.381

Acetate uptake rate

   

0.33

0.22

≈ 0.161

Propionate uptake rate

-

-

-

0.34

0.23

≈ 0.221

 

Carbon and oxygen flux distribution (%)

Carbon converted to growth

0.7

3.3

15.0

2.0

3.3

8.7

Carbon converted to CO 2

25.7

35.0

37.8

20.0

28.1

50.1

Oxygen consumed per carbon

16.5

27.3

19.8

14.7

24.3

-

Carbon converted to PHA

73.6

61.7

47.2

78.0

68.6

41.2

Carbon converted to Ac-CoA*

73.6

61.7

47.2

45.2

39.5

36.9

Carbon converted to Propionyl-CoA*

-

-

-

32.8

29.1

4.3

  1. 1Approximate rates estimated from experimental results