Table 8 Central metabolism - parameters.

k _{r 1,3,4,9}

rate constants for the network functions h₁, h₃, h₄ and h₉

1/μ mol²/s

assumed

k _{r 2}

rate constant for the network function h₂

1/μ mol/s

assumed

V_pdh

maximal velocity of the reaction involving pyruvate dehydro-genase (PDH)

25μ mol

[67]

concentrations of AcCoA at half saturation of the reaction involving PDH

0.2μ mol

[67]

V_keto

maximal velocity of isocitrate dehydrogenase reaction

100μ mol

[68]

ATP levels at half saturation of the isocitrate dehydrogenase reaction

7μ mol/g

[25, 66, 69]

V_mal

maximal velocity of the malate-pyruvate pathway, must be smaller than k_oxal to maintain regulation. Estimated from [70]

0.1 μ mol

estimated

oxaloacetate levels at half saturation of the malate-pyruvate pathway

0.04 nmol/g

[71]

V_oxal

maximal velocity of the pyruvate-oxaloacetate pathway

5 μ mol

[58]

AcCoA levels at half saturation in the pyruvate-oxaloacetate pathway

3μ mol/g

[58]

k _tca

rate constant in the TCA cycle

10/s/μ mol

[69]

k _citr

Half saturation constant in citrate aconitase reaction

1.4/s

[72]

k _iso

rate constant of citrate aconitase reversible reaction

10/s

[73]

k _oxal

rate constant of malate to oxaloacetate reaction

1.4/s/μ mol

[70]

k _mal

rate constant of oxaloacetate to malate reaction

2.8/s/μ mol²

[70]

k _cyt

rate constant of citrate transport from the matrix to the cytosol (by tricarboxylate carriers), estimated from [74], must be slower than isocitrate dehydrogenase reaction

1/s/μ mol

estimated