Figure 8

Metabolic flux distributions in G. metallireducens by in silico genome-scale modeling. G. metallireducens growth with acetate as electron donor and Fe(III) as electron acceptor was simulated with under 21 mmol/gdw/h of acetate uptake flux with acetate as the limiting factor, the same as a ¹³C isotopomer labeling flux analysis. The predicted flux distributions by the genome-scale modeling (upper numbers) were compared to the flux distributions determined in the [1-¹³C]acetate labeling experiments (lower numbers). Dotted arrows indicated the pathways involving some energy-inefficient reactions that had no flux under this condition.