From: Proteomic and network analysis characterize stage-specific metabolism in Trypanosoma cruzi
Organism and stage | Experimental Method [ref] | Experimental Target | Model reaction(s) constrained | Finding from literature | Full model prediction | Epimastigote model prediction |
---|---|---|---|---|---|---|
bloodstream T. brucei*** | drug-2-hydroxybenzaldehyde-5-phosphate [37] | fructose-1,6-bisphosphate aldolase | FBAg | lethal | lethal | lethal |
T. cruzi amastigotes | drug- 6-phosphogluconate analogues [38] | phosphogluconate dehydrogenase | PGDH | lethal | lethal | lethal |
T. cruzi amastigotes | drug- adenosine analogs [39] | glyceraldehyde-3-phosphate dehydrogenase | GAPDg | lethal | lethal | lethal |
T. cruzi epimastigotes | drug- bisphosphonates [40] | hexokinase | HEXg, GLUKg | lethal | lethal | lethal |
T. cruzi epimastigotes | monoclonal antibody [41] | triosephosphate isomerase | TPIg | lethal | nonlethal | lethal |
T. cruzi epimastigotes | drug- peroxynitrite [42] | succinate dehydrogenase and fumarate reductase* | SUCD1rm**, FRDm, FRDgr | lethal | nonlethal | lethal |
bloodstream T. brucei | RNAi [43] | phosphofructokinase | PFKg | lethal | lethal | lethal |
bloodstream T. brucei | RNAi [43] | phosphoglycerate mutase | PGM | lethal | lethal | lethal |
bloodstream T. brucei | RNAi [43] | enolase | ENO | lethal | lethal | lethal |
procyclic T. brucei | RNAi [44] | malic enzyme | ME1x, ME1m | lethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [45] | e1 alpha subunit of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase | PDHam1m, AKGDam1 | lethal | nonlethal | lethal |
bloodstream T. brucei | RNAi [43] | pyruvate kinase | PYK | lethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [45] | succinyl-CoA synthetase (succinate CoA ligase) | SUCOASm | lethal | nonlethal | nonlethal |
L. donovanni promastigotes | nontargeted gene disruption [46] | adenosine kinase | ADK1g | nonlethal | nonlethal | nonlethal |
bloodstream T. brucei | RNAi [47] | alanine aminotransferase | ALATA_L, ALATA_Lm | nonlethal | nonlethal | nonlethal |
bloodstream T. brucei | RNAi [47] | cytochrome C oxidase subunit IV | CYOO6m | nonlethal | nonlethal | nonlethal |
bloodstream T. brucei | RNAi [47] | glycerol-3-phosphate dehydrogenase (FAD) | G3PDcm | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [45] | e1 subunit of 2-oxoglutarate dehydrogenase | AKGDe1 | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [44] | F1 complex of ATP synthase | ATPSm | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [48] | glycosomal NADH-dependent fumarate reductase | FRDgr | nonlethal | nonlethal | lethal |
procyclic T. brucei | RNAi [44] | mitochondrial fumarate reductase | FRDm | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [45] | e1 alpha subunit of pyruvate dehydrogenase | PDHam1m | nonlethal | nonlethal | lethal |
procyclic T. brucei | RNAi [45] | e1 alpha subunit of pyruvate dehydrogenase, succinate dehydrogenase | PDHam1m, SUCD1rm | nonlethal | nonlethal | lethal |
procyclic T. brucei | RNAi [44] | phosphoenolpyruvate carboxykinase | PPCKg | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [44] | proline oxidase | PRO1xm | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | RNAi [45] | succinate dehydrogenase | SUCD1rm | nonlethal | nonlethal | nonlethal |
bloodstream T. brucei | targeted gene disruption [49] | aconitase | ACONTm | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | targeted gene disruption [50] | acetyl:succinate CoA-transferase | ASCTmr | nonlethal | nonlethal | nonlethal |
procyclic T. brucei | targeted gene disruption [51] | pyruvate phosphate dikinase | PPDKg | nonlethal | nonlethal | nonlethal |